Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector.

ABSTRACT:

Background

Due to its high expression capability, recombination of Escherichia coli and pET vector has become the bioengineering preferred expression system. Because β-lactamases mediate bacterial antimicrobial resistance, these enzymes have a substantial clinical impact. Using the E. coli expression system, several kinds of β-lactamases have been produced. However, previous studies have been focused on characterizing target β-lactamases, and the effects of cultivation and induction conditions on the expression efficiency of target enzymes were not addressed.

Results

Using pET-28a as the cloning vector and E. coli BL21(DE3) as the expression host, this study originally elucidated the effects of IPTG concentration, culture temperature, induction time, and restriction sites on

SUBMITTER: Li L 

PROVIDER: S-EPMC9686023 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature