Mediating oxidative stress enhances α-ionone biosynthesis and strain robustness during process scaling up.

ABSTRACT:

Background

α-Ionone is highly valued in cosmetics and perfumery with a global usage of 100-1000 tons per year. Metabolic engineering by microbial fermentation offers a promising way to produce natural (R)-α-ionone in a cost-effective manner. Apart from optimizing the metabolic pathways, the approach is also highly dependent on generating a robust strain which retains productivity during the scale-up process. To our knowledge, no study has investigated strain robustness while increasing α-ionone yield.

Results

Built on our previous work, here, we further increased α-ionone yield to 11.4 mg/L/OD in 1 mL tubes by overexpressing the bottleneck dioxygenase CCD1 and re-engineering the pathway, which is  > 65% enhancement as compared to our previously best strain. However, the yiel

SUBMITTER: Huang CN 

PROVIDER: S-EPMC9686065 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature