Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO.

ABSTRACT:

Background

Ethyl acetate is a bulk chemical traditionally produced via energy intensive chemical esterification. Microbial production of this compound offers promise as a more sustainable alternative process. So far, efforts have focused on using sugar-based feedstocks for microbial ester production, but extension to one-carbon substrates, such as CO and CO₂/H₂, is desirable. Acetogens present a promising microbial platform for the production of ethyl esters from these one-carbon substrates.

Results

We engineered the acetogen C. autoethanogenum to produce ethyl acetate from CO by heterologous expression of an alcohol acetyltransferase (AAT), which catalyzes the formation of ethyl acetate from acetyl-CoA and ethanol. Two AATs, Eat1 from Kluyveromyces mar

SUBMITTER: Dykstra JC 

PROVIDER: S-EPMC9686113 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature