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Bioassay-guided isolation of Fenghuang Dancong tea constituents with α-glucosidase inhibition activities.

ABSTRACT: An α-glucosidase inhibition assay showed the ethanolic extract of Fenghuang Dancong tea had potential α-glucosidase inhibitory activity. The most bioactive fraction, which was obtained via bioassay-guided isolation of the extract, was further purified to create five compounds, including three novel compounds (1-3). These compounds were analyzed and identified in detail using high-resolution-mass spectrometry and extensive one-dimensional and two-dimensional NMR spectroscopy experiments. Among the compounds, compound 1 contained cis double bonds and showed the strongest α-glucosidase inhibitory activity with an IC₅₀ value of 7.51 μM, which is significantly lower than that of compound 2 with trans double bonds. Enzyme kinetic experiments showed that 1 was a reversible non-competitive α-glucosidase inhibitor.

SUBMITTER: Zhou H

PROVIDER: S-EPMC9686339 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Bioassay-guided isolation of Fenghuang Dancong tea constituents with α-glucosidase inhibition activities.

Zhou Hua H Liao Jin J Ou Juanying J Lin Jiayu J Zheng Jie J Li Yifei Y Ou Shiyi S Liu Fu F

Frontiers in nutrition 20221110

An α-glucosidase inhibition assay showed the ethanolic extract of Fenghuang Dancong tea had potential α-glucosidase inhibitory activity. The most bioactive fraction, which was obtained via bioassay-guided isolation of the extract, was further purified to create five compounds, including three novel compounds (1-3). These compounds were analyzed and identified in detail using high-resolution-mass spectrometry and extensive one-dimensional and two-dimensional NMR spectroscopy ...[more]

PMID: 36438778

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Bioassay-guided isolation of Fenghuang Dancong tea constituents with α-glucosidase inhibition activities.

Publications

Bioassay-guided isolation of Fenghuang Dancong tea constituents with α-glucosidase inhibition activities.

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