GPER limits adverse changes to Ca²⁺ signalling and arrhythmogenic activity in ovariectomised guinea pig cardiomyocytes.

ABSTRACT: Background: The increased risk of post-menopausal women developing abnormalities of heart function emphasises the requirement to understand the effect of declining oestrogen levels on cardiac electrophysiology and structure, and investigate possible therapeutic targets, namely the G protein-coupled oestrogen receptor 1 (GPER). Methods: Female guinea pigs underwent sham or ovariectomy (OVx) surgeries. Cardiomyocytes were isolated 150-days post-operatively. Membrane structure was assessed using di-8-ANEPPs staining and scanning ion conductance microscopy. Imunnohistochemistry (IHC) determined the localisation of oestrogen receptors. The effect of GPER activation on excitation-contraction coupling mechanisms were assessed using electrophysiological and fluorescence techniques. D

SUBMITTER: Francis AJ 

PROVIDER: S-EPMC9686394 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature