A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes.

ABSTRACT: IncL/M broad-host-range conjugative plasmids are involved in the global spread of bla_OXA-48 and the emergence of bla_NDM-1. The aim of this study was to evaluate the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The conjugation rate and fitness cost of IncM2 and IncL plasmids encoding these carbapenemase genes were tested using a variety of host bacteria. Genomic analysis of uropathogenic Escherichia coli SAP1756 revealed that bla_NDM-1 was encoded on an IncM2 plasmid, which also harboured bla_TEM-1, ble_MBL and sul1 and was highly similar to plasmids isolated from the same geographical area. Conjugation experiments demonstrated

SUBMITTER: Getino M 

PROVIDER: S-EPMC9686884 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature