Microscale Quantification of the Inhibition of Neuraminidase Using Capillary Nanogel Electrophoresis.

ABSTRACT: Neuraminidase inhibitors modulate infections that involve sialic acids, making quantitative analyses of this inhibitory effect important for selecting and designing potential therapeutics. An automated nanogel capillary electrophoresis system is developed that integrates a 5 nL enzyme inhibition reaction in line with a 5 min separation-based assay of the enzymatic product to quantify inhibition as the half maximal inhibitory concentration (IC₅₀) and inhibitor constant (K_i). A neuraminidase enzyme from Clostridium perfringens is non-covalently immobilized in a thermally tunable nanogel positioned in the thermally controlled region of the capillary by increasing the capillary temperature to 37 °C. Aqueous inhibitor solutions are loaded into the capillary d

SUBMITTER: Casto-Boggess LD 

PROVIDER: S-EPMC9686991 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature