CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth.

ABSTRACT: There is a growing amount of data uncovering the cellular diversity of the pulmonary circulation and mechanisms governing vascular repair after injury. However, the molecular and cellular mechanisms contributing to the morphogenesis and growth of the pulmonary vasculature during embryonic development are less clear. Importantly, deficits in vascular development lead to significant pediatric lung diseases, indicating a need to uncover fetal programs promoting vascular growth. To address this, we used a transgenic mouse reporter for expression of Cxcl12, an arterial endothelial hallmark gene, and performed single-cell RNA sequencing on isolated Cxcl12-DsRed+ endothelium to assess cellular heterogeneity within pulmonary endothelium. Combining cell annotation with gene ontology and histologica

SUBMITTER: Chandrasekaran P 

PROVIDER: S-EPMC9687018 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature