A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis.

ABSTRACT: Interstitial flow plays a significant role in vascular system development, mainly including angiogenesis and vasculogenesis. However, compared to angiogenesis, the effect of interstitial flow on vasculogenesis is less explored. Current in vitro models for investigating the effect of interstitial flow on vasculogenesis heavily rely on microfluidic chips, which require microfluidic expertise and facilities, and may not be accessible to biological labs. Here, we proposed a facile approach to building perfusable vascular networks through the self-assembly of endothelial cells in a modified transwell format and investigated the effect of interstitial flow on vasculogenesis. We found that the effect of interstitial flow on vasculogenesis was closely related to the existence of VEGF and fibroblas

SUBMITTER: Deng P 

PROVIDER: S-EPMC9687519 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature