Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering.

ABSTRACT:

Background

In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and reseeded them with allogeneic porcine mesenchymal stromal cells (pMSCs) to understand whether extracellular matrix (ECM) can influence and/or promote differentiation into hepatocyte-like cells (HLCs).

Methods

After decellularisation with SDS, the integrity of ECM-scaffolds was examined by histological staining, immunofluorescence and scanning electron microscope. DNA quantification was used to assess decellularisation. pMSCs were plated on scaffolds by static seeding and maintaine

SUBMITTER: Croce S 

PROVIDER: S-EPMC9687774 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature