Assessing the Formation of Purine Lesions in Mitochondrial DNA of Cockayne Syndrome Cells.

ABSTRACT: Mitochondrial (mt) DNA and nuclear (n) DNA have known structures and roles in cells; however, they are rarely compared under specific conditions such as oxidative or degenerative environments that can create damage to the DNA base moieties. Six purine lesions were ascertained in the mtDNA of wild type (wt) CSA (CS3BE-wtCSA) and wtCSB (CS1AN-wtCSB) cells and defective counterparts CS3BE and CS1AN in comparison with the corresponding total (t) DNA (t = n + mt). In particular, the four 5',8-cyclopurine (cPu) and the two 8-oxo-purine (8-oxo-Pu) lesions were accurately quantified by LC-MS/MS analysis using isotopomeric internal standards after an enzymatic digestion procedure. The 8-oxo-Pu levels were found to be in the range of 25-50 lesions/10⁷ nucleotides in both the mtDNA and tDN

SUBMITTER: Chatgilialoglu C 

PROVIDER: S-EPMC9687895 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature