Rapid Bacteria Detection from Patients' Blood Bypassing Classical Bacterial Culturing.

ABSTRACT: Sepsis is a life-threatening condition mostly caused by a bacterial infection resulting in inflammatory reaction and organ dysfunction if not treated effectively. Rapid identification of the causing bacterial pathogen already in the early stage of bacteremia is therefore vital. Current technologies still rely on time-consuming procedures including bacterial culturing up to 72 h. Our approach is based on ultra-rapid and highly sensitive nanomechanical sensor arrays. In measurements we observe two clearly distinguishable distributions consisting of samples with bacteria and without bacteria respectively. Compressive surface stress indicates the presence of bacteria. For this proof-of-concept, we extracted total RNA from EDTA whole blood samples from patients with blood-culture-confirmed bact

SUBMITTER: Huber F 

PROVIDER: S-EPMC9688106 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature