Involvement of DAAO Overexpression in Delayed Hippocampal Neuronal Death.

ABSTRACT:

Background

D-amino acid oxidase (DAAO) is a flavoenzyme that specifically catalyzes the deamination of many neutral and basic D-amino acids. This study aims to explore the pathological increment of hippocampal DAAO and its potential relationship with delayed hippocampal neuronal death.

Methods

Ischemia-reperfusion was induced in mice through middle cerebral artery occlusion (MCAO). Neurological deficit scores and hippocampal neuronal death were assessed in MCAO mice. Immunofluorescent staining was applied to identify activated astrocytes and evaluate DAAO expression. TUNEL and Nissl staining were utilized to identify cell apoptosis of hippocampal neurons.

Results

Hippocampal astrocytic DAAO was strikingly increased following ischemic stroke, with the greatest increase

SUBMITTER: Liu H 

PROVIDER: S-EPMC9688509 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature