The Molecular Signature of Human Testicular Peritubular Cells Revealed by Single-Cell Analysis.

ABSTRACT: Peritubular cells of the human testis form a small compartment surrounding the seminiferous tubules. They are crucial for sperm transport, and they emerge as contributors to the spermatogonial stem cell niche. They are among the least known cell types of the human body. We employed single-cell RNA sequencing of cultured human testicular peritubular cells (HTPCs), which had been isolated from testicular samples of donors with normal spermatogenesis. The significant overlap between our results and recently published ex vivo data indicates that HTPCs are a highly adequate cellular model to define and study these cells. Thus, based on the expression of several markers, HTPCs can be classified as testicular smooth muscle cells. Small differences between the in vivo/in vitro expressed genes may

SUBMITTER: Liebich A 

PROVIDER: S-EPMC9688777 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature