Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional study.

ABSTRACT:

Background

The transmission of monkeypox virus occurs through direct contact, but transmission through saliva or exhaled droplets and aerosols has not yet been investigated. We aimed to assess the presence of monkeypox virus DNA and infectious virus in saliva samples and droplets and aerosols exhaled from patients infected with monkeypox virus.

Methods

We did a cross-sectional study in patients with monkeypox confirmed by PCR who attended two health centres in Madrid, Spain. For each patient, we collected samples of saliva, exhaled droplets within a mask, and aerosols captured by air filtration through newly developed nanofiber filters. We evaluated the presence of monkeypox virus in the samples by viral DNA detection by quantitative PCR (qPCR) and isolation of infectious vi

SUBMITTER: Hernaez B 

PROVIDER: S-EPMC9691259 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature