A Knock-In Mouse Model of Thymoma With the GTF2I L424H Mutation.

ABSTRACT:

Introduction

The pathogenesis of thymic epithelial tumors remains largely unknown. We previously identified GTF2I L424H as the most frequently recurrent mutation in thymic epithelial tumors. Nevertheless, the precise role of this mutation in tumorigenesis of thymic epithelial cells is unclear.

Methods

To investigate the role of GTF2I L424H mutation in thymic epithelial cells in vivo, we generated and characterized a mouse model in which the Gtf2i L424H mutation was conditionally knocked-in in the Foxn1+ thymic epithelial cells. Digital spatial profiling was performed on thymomas and normal thymic tissues with GeoMx-mouse whole transcriptome atlas. Immunohistochemistry staining was performed using both mouse tissues and human thymic epithelial tumors.

Results

We observ

SUBMITTER: He Y 

PROVIDER: S-EPMC9691559 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature