Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility - a pilot study.

ABSTRACT: Unexplained infertility affects about one-third of infertile couples and is defined as the failure to identify the cause of infertility despite extensive evaluation of the male and female partners. Therefore, there is a need for a multiparametric approach to study sperm function. Recently, we developed a Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) assay to determine sperm centriole quality. Here, we perform a pilot study of sperm from 10 fertile men and 10 men in couples with unexplained infertility, using three centriolar biomarkers measured at three sperm locations from two sperm fractions, representing high and low sperm quality. We found that FRAC can identify men from couples with unexplained infertility as the likely source of infertility. Higher quality fracti

SUBMITTER: Jaiswal A 

PROVIDER: S-EPMC9692136 | biostudies-literature | 2022 Jun-Aug

REPOSITORIES: biostudies-literature