Measuring Structural Changes in Cytochrome c under Crowded Conditions Using In Vitro and In Silico Approaches.

ABSTRACT: It is known from in vitro studies that macromolecular crowding in the cell effects protein structure, stability and function; but predictive studies are relatively unexplored. There are few reports where the effect of various crowder mixtures has been exploited to discern their combined effect on the structural stability of proteins. These studies are more significant because their effect can mimicked with in vivo conditions, where the environment is heterogeneous. Effects of two crowders, polyethylene glycol (PEG 400 Da), and its monomer ethylene glycol (EG) alone and in mixture on the structural stability of cytochrome c (cyt c) were determined using various spectroscopic and bioinformatics tools. The main conclusions of our study are (i) the monomer EG has a kosmotropic ef

SUBMITTER: Parray ZA 

PROVIDER: S-EPMC9692323 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature