Characterization of a Glycolipid Synthase Producing α-Galactosylceramide in Bacteroides fragilis.

ABSTRACT: Glycolipids are complex molecules involved in important cellular processes. Among them, the glycosphingolipid α-galactosylceramide has proven to be of interest in biomedicine for its immunostimulatory capabilities. Given its structural requirements, the use of ceramide glycosyltransferase enzymes capable of synthesizing this molecule under in vivo or in vitro conditions is a potential production strategy. Several GT4 enzymes from Bacteroides fragilis were considered as potential candidates in addition to the known BF9343_3149, but only this one showed glycolipid synthase activity. The enzyme was expressed as a SUMO fusion protein to produce soluble protein. It is a non-processive glycosyltransferase that prefers UDP-Gal over UDP-Glc as a donor substrate, and maximum activity was fou

SUBMITTER: Caballe M 

PROVIDER: S-EPMC9692976 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature