Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.

ABSTRACT: The creation of oral microcosms with reproducible composition is important for developing model systems of the oral microbiome. However, oral microbiomes vary substantially across individuals. To derive a reproducible composition from inocula sourced from different individuals, we tested whether selective conditions from cold storage and culturing in defined media would generate a reproducible community composition despite individual variations. In this pilot study, we collected dental plaque scrapings from three individuals, inoculated media under anaerobic conditions, and characterized the bacterial community compositions after cold storage and subsequent propagation in liquid media. Harvested cultures were extracted and bacterial composition was determined by 16S rRNA gene amplicon sequ

SUBMITTER: Zhou B 

PROVIDER: S-EPMC9693135 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature