Degradation-Suppressed Cocoonase for Investigating the Propeptide-Mediated Activation Mechanism.

ABSTRACT: Cocoonase is folded in the form of a zymogen precursor protein (prococoonase) with the assistance of the propeptide region. To investigate the role of the propeptide sequence on the disulfide-coupled folding of cocoonase and prococoonase, the amino acid residues at the degradation sites during the refolding and auto-processing reactions were determined by mass spectrometric analyses and were mutated to suppress the numerous degradation reactions that occur during the reactions. In addition, the Lys⁸ residue at the propeptide region was also mutated to estimate whether the entire sequence is absolutely required for the activation of cocoonase. Finally, a degradation-suppressed [K8D,K63G,K131G,K133A]-proCCN protein was prepared and was found to refold readily without significant d

SUBMITTER: Sakata N 

PROVIDER: S-EPMC9693254 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature