Report and Comparative Genomics of an NDM-5-Producing Escherichia coli in a Portuguese Hospital: Complex Class 1 Integrons as Important Players in bla_NDM Spread.

ABSTRACT:

Background

New Delhi metallo-beta-lactamase (NDM) has been spreading across the globe, but the causes of its success are poorly understood. We characterized a bla_NDM-5-positive Escherichia coli strain from a Portuguese hospital and conducted comparative genomic analyses to understand the role of clonal background and horizontal gene transfer in bla_NDM-5 dissemination.

Methods

After bla_NDM PCR screening and genome sequencing, Ec355340 was subjected to mating, transformation, and plasmid curing assays and MICs determination for several antibiotics. Comparison with data compiled from public databases was performed.

Results

bla_NDM-5 was in a complex integron co-located in a FIB-FII plasmid (pEc

SUBMITTER: Tavares RDS 

PROVIDER: S-EPMC9693474 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature