Isolated Effects of Plasma Freezing versus Thawing on Metabolite Stability.

ABSTRACT: Freezing and thawing plasma samples is known to perturb metabolite stability. However, no study has systematically tested how different freezing and thawing methods affect plasma metabolite levels. The objective of this study was to isolate the effects of freezing from thawing on mouse plasma metabolite levels, by comparing a matrix of freezing and thawing conditions through 10 freeze-thaw cycles. We tested freezing with liquid nitrogen (LN₂), at -80 °C, or at -20 °C, and thawing quickly in room temperature water or slowly on ice. Plasma samples were extracted and the relative abundance of 87 metabolites was obtained via liquid chromatography-mass spectrometry (LC-MS). Observed changes in metabolite abundance by treatment group correlated with the amount of time it took for samp

SUBMITTER: Buchanan JL 

PROVIDER: S-EPMC9693613 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature