Salmonella typhimurium virulence genes are induced upon bacterial invasion into phagocytic and nonphagocytic cells.

ABSTRACT: Survival and growth of salmonellae within host cells are important aspects of bacterial virulence. We have developed an assay to identify Salmonella typhimurium genes that are induced inside Salmonella-containing vacuoles within macrophage and epithelial cells. A promoterless luciferase gene cassette was inserted randomly into the Salmonella chromosome, and the resulting mutants were screened for genes upregulated in intracellular bacteria compared to extracellular bacteria. We identified four genes in S. typhimurium that were upregulated upon bacterial invasion of both phagocytic and nonphagocytic cells. Expression of these genes was not induced by factors secreted by host cells or media alone. All four genes were induced at early time points (2 to 4 h) postinvasion and continued to be up

SUBMITTER: Pfeifer CG 

PROVIDER: S-EPMC96943 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature