Leucine and Arginine Availability Modulate Mouse Embryonic Stem Cell Proliferation and Metabolism.

ABSTRACT: Amino acids are crucial nutrients involved in several cellular and physiological processes, including fertilization and early embryo development. In particular, Leucine and Arginine have been shown to stimulate implantation, as lack of both in a blastocyst culture system is able to induce a dormant state in embryos. The aim of this work was to evaluate the effects of Leucine and Arginine withdrawal on pluripotent mouse embryonic stem cell status, notably, their growth, self-renewal, as well as glycolytic and oxidative metabolism. Our results show that the absence of both Leucine and Arginine does not affect mouse embryonic stem cell pluripotency, while reducing cell proliferation through cell-cycle arrest. Importantly, these effects are not related to Leukemia Inhibitory Factor (LIF) and a

SUBMITTER: Correia B 

PROVIDER: S-EPMC9694364 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature