Dual-RNAseq Analysis Unravels Virus-Host Interactions of MetSV and Methanosarcina mazei.

ABSTRACT: Methanosarcina spherical virus (MetSV), infecting Methanosarcina species, encodes 22 genes, but their role in the infection process in combination with host genes has remained unknown. To study the infection process in detail, infected and uninfected M. mazei cultures were compared using dual-RNAseq, qRT-PCRs, and transmission electron microscopy (TEM). The transcriptome analysis strongly indicates a combined role of virus and host genes in replication, virus assembly, and lysis. Thereby, 285 host and virus genes were significantly regulated. Within these 285 regulated genes, a network of the viral polymerase, MetSVORF6, MetSVORF5, MetSVORF2, and the host genes encoding NrdD, NrdG, a CDC48 family protein, and a SSB protein with a role in viral repl

SUBMITTER: Gehlert FO 

PROVIDER: S-EPMC9694453 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature