Limitations of Tamoxifen Application for In Vivo Genome Editing Using Cre/ER^T2 System.

ABSTRACT: Inducible Cre-dependent systems are frequently used to produce both conditional knockouts and transgenic mice with regulated expression of the gene of interest. Induction can be achieved by doxycycline-dependent transcription of the wild type gene or OH-tamoxifen-dependent nuclear translocation of the chimeric Cre/ER^T2 protein. However, both of these activation strategies have some limitations. We analyzed the efficiency of knockout in different tissues and found out that it correlates with the concentration of the hydroxytamoxifen and endoxifen-the active metabolites of tamoxifen-measured by LC-MS in these tissues. We also describe two cases of Cdk8^{floxed/floxed}/Rosa-Cre-ER^T2 mice tamoxifen-induced knockout limitations. In the first case, the standard sche

SUBMITTER: Ilchuk LA 

PROVIDER: S-EPMC9694728 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature