The Contribution of Phospholipase A₂ and Metalloproteinases to the Synergistic Action of Viper Venom on the Bioenergetic Profile of Vero Cells.

ABSTRACT: Increasing concern about the use of animal models has stimulated the development of in vitro cell culture models for analysis of the biological effects of snake venoms. However, the complexity of animal venoms and the extreme synergy of the venom components during envenomation calls for critical review and analysis. The epithelium is a primary target for injected viper venom's toxic substances, and therefore, is a focus in modern toxinology. We used the Vero epithelial cell line as a model to compare the actions of a crude Macrovipera lebetina obtusa (Levantine viper) venom with the actions of the same venom with two key enzymatic components inhibited (specifically, phospholipase A2 (PLA2) and metalloproteinases) in the bioenergetic cellular response, i.e., oxygen uptake and reactiv

SUBMITTER: Ayvazyan N 

PROVIDER: S-EPMC9695613 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature