RSAD2 Is an Effective Target for High-Yield Vaccine Production in MDCK Cells.

ABSTRACT: Increasingly, attention has focused on improving vaccine production in cells using gene editing technology to specifically modify key virus regulation-related genes to promote virus replication. In this study, we used DIA proteomics analysis technology to compare protein expression differences between two groups of MDCK cells: uninfected and influenza A virus (IAV) H1N1-infected cells 16 h post infection (MOI = 0.01). Initially, 266 differentially expressed proteins were detected after infection, 157 of which were upregulated and 109 were downregulated. We screened these proteins to 23 genes related to antiviral innate immunity regulation based on functional annotation database analysis and verified the mRNA expression of these genes using qPCR. Combining our results with published literat

SUBMITTER: Qiao Z 

PROVIDER: S-EPMC9695692 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature