Detection of Highly Poisonous Nerium oleander Using Quantitative Real-Time PCR with Specific Primers.

ABSTRACT: Nerium oleander is one of the most poisonous plants, and its accidental ingestion has frequently occurred in humans and livestock. It is vital to develop a rapid and accurate identification method for the timely rescue of oleander-poisoned patients and the investigation of poisoning cases. In this study, a specific and highly sensitive quantitative real-time PCR (qPCR)-based method was developed to identify oleander in mixture systems and simulated forensic specimens (SFS). First, a new pair of oleander-specific primers, JZT-BF/BR, was designed and validated. Then, a qPCR method was developed using the primers, and its detective sensitivity was examined. The results showed that JZT-BF/BR could specifically identify oleander in forage and food mixtures, and qPCR was capable of accura

SUBMITTER: Bai X 

PROVIDER: S-EPMC9696062 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature