Bioimaging of Lysosomes with a BODIPY pH-Dependent Fluorescent Probe.

ABSTRACT: Fluorescence-based probes represent a powerful tool for noninvasive imaging of living systems in real time and with a high temporal and spatial resolution. Amongst several known fluorophores, 3-difluoroborodipyrromethene (BODIPY) derivatives have become a cornerstone for innovative fluorescent labelling applications, mainly due to their advantageous features including their facile synthesis, structural versatility and exceptional photophysical properties. In this context, we report a BODIPY-based fluorescent probe for imaging of lysosomes in living cells. The BODIPY derivative displayed a remarkable fluorescence enhancement at low pH values with a pK_a* of 3.1. In vitro studies by confocal microscopy in HeLa cells demonstrated that the compound was able to permeate cell membrane

SUBMITTER: Goncalves RCR 

PROVIDER: S-EPMC9696654 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature