Associations between NK Cells in Different Immune Organs and Cellular SIV DNA and RNA in Regional HLADR^- CD4⁺ T Cells in Chronically SIV_mac239-Infected, Treatment-Naive Rhesus Macaques.

ABSTRACT: With the development of NK cell-directed therapeutic strategies, the actual effect of NK cells on the cellular SIV DNA levels of the virus in SIV-infected macaques in vivo remains unclear. In this study, five chronically SIV_mac239-infected, treatment-naïve rhesus macaques were euthanized, and the blood, spleen, pararectal/paracolonic lymph nodes (PaLNs), and axillary lymph nodes (ALNs) were collected. The distributional, phenotypic, and functional profiles of NK cells were detected by flow cytometry. The highest frequency of NK cells was found in PBMC, followed by the spleen, while only 0~0.5% were found in LNs. Peripheral NK cells also exhibited higher cytotoxic potential (CD56^- CD16⁺ NK subsets) and IFN-γ-producing capacity but low PD-1 and Tim-3 levels t

SUBMITTER: Li X 

PROVIDER: S-EPMC9697022 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature