Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing.

ABSTRACT: The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool. In the current study, we utilized deep sequencing to characterize the widely used Ph.DTM-12 phage display peptide library (New England Biolabs). The next-generation sequencing (NGS) data indicated the presence of stop codons and a high abundance of wild-type clones in the naïve library, which collectively result in a reduced effective size of the library. The analysis of the DNA sequence logo and global and position-specific frequency of amino acids demonstrated significant bias in the nucleotide and amino acid composition of the library inserts. Principal component

SUBMITTER: Sloth AB 

PROVIDER: S-EPMC9697088 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature