Obtaining Highly Active Catalytic Antibodies Capable of Enzymatically Cleaving Antigens.

ABSTRACT: A catalytic antibody has multiple functions compared with a monoclonal antibody because it possesses unique features to digest antigens enzymatically. Therefore, many catalytic antibodies, including their subunits, have been produced since 1989. The catalytic activities often depend on the preparation methods and conditions. In order to elicit the high catalytic activity of the antibodies, the most preferable methods and conditions, which can be generally applicable, must be explored. Based on this view, systematic experiments using two catalytic antibody light chains, #7TR and H34, were performed by varying the purification methods, pH, and chemical reagents. The experimental results obtained by peptidase activity tests and kinetic analysis, revealed that the light chain's high catalytic

SUBMITTER: Nonaka T 

PROVIDER: S-EPMC9697424 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature