Micrococcus porci sp. nov., Isolated from Feces of Black Pig (Sus scrofa).

ABSTRACT: An aerobic bacterium, designated as strain KD337-16^T, was isolated from the fecal samples of a black pig. It exhibited spherical, non-motile and non-spore-forming, Gram-positive cells. KD337-16^T was identified as a member of the genus Micrococcus through 16S rRNA gene sequencing, and its closest relatives were found to be Micrococcus endophyticus YIM 56238^T (99.5% similarity), Micrococcus luteus NCTC 2665^T (99.1%), Micrococcus yunnanensis YIM 65004^T (99.1%), Micrococcus aloeverae AE-6^T (99.1%), Micrococcus antarcticus T2^T (98.9%), and Micrococcus flavus LW4^T (98.7%). Phylogenomic trees were constructed, and strain KD337-16^T was found to form its own cluster as an independent lineage of M. flavus LW4^T. Between KD337-16^T and its close relatives, the average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization were below the respective species delineation thresholds at 82.1-86.6%, 78.1-86.1%, and 24.4-34.9%. The major cellular fatty acids and polar lipids were anteiso-C_15:0 and iso-C_15:0, and DPG and PG, respectively. The predominant menaquinone was MK-8(H₂). Taken together, the results indicate that strain KD337-16^T is a novel species of the genus Micrococcus, for which the name Micrococcus porci sp. nov. is proposed. The type strain is KD337-16^T (=BCRC 81318^T = NBRC 115578^T).

SUBMITTER: Lee AY 

PROVIDER: S-EPMC9697426 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature