Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model.

ABSTRACT: We utilised a ground-based microgravity hindlimb unloading (HU) mouse model to elucidate the gut microbiota bacterial changes in mice under a simulated microgravity environment. Four-month-old, male C57/Bl6 mice were randomly divided into ground-based controls and the HU groups and kept under controlled environmental conditions. For the microgravity environment, the mice were suspended in special cages individually for 20 days. At the end of the suspension, the mice were sacrificed; gut dissections were performed, followed by a metagenomic analysis of bacterial species, which was carried out by extracting DNA and 16S rRNA analysis. The results revealed that the gut bacterial communities of mice under gravity and microgravity were different. Notably, our findings revealed differences in the

SUBMITTER: Siddiqui R 

PROVIDER: S-EPMC9698145 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature