Airway Bacterial Biodiversity in Exhaled Breath Condensates of Asthmatic Children—Does It Differ from the Healthy Ones?

ABSTRACT: Asthma etiopathology is still not fully determined. One of its possible causes can be found in airway microbiome dysbiosis. The study’s purpose was to determine whether there are any significant differences in the bacterial microbiome diversity of lower airways microbiota of asthmatic children, since knowledge of this topic is very scarce. To the authors’ knowledge, this is the first research using exhaled breath condensates in children’s lower airways for bacterial assessment. Exhaled breath condensates (EBC) and oropharyngeal swabs were obtained from pediatric asthmatic patients and a healthy group (n = 38, 19 vs. 19). The microbial assessment was conducted through genetic material PCR amplification, followed by bacterial 16S rRNA amplicon sequencing. Collected data were analyzed, in ter

SUBMITTER: Bar K 

PROVIDER: S-EPMC9698396 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature