Insight into the Interaction Mechanism of HSA with Aztreonam: A Multispectroscopic and Computational Approach.

ABSTRACT: Aztreonam is a Gram-negative bacteria-targeting synthetic monobactam antibiotic. Human serum albumin (HSA) plays an important role in the transference of pharmaceuticals, hormones, and fatty acids, along with other compounds, determining their biodistribution and physiological fate. Using several biophysical and in silico approaches, we studied the interaction of aztreonam with HSA under physiological environments in this study. Results confirm the formation of HSA-aztreonam complex where aztreonam showed moderate affinity towards HSA. A static mode of quenching was confirmed from the steady state fluorescence data. FRET findings also showed that there was a significant feasibility of energy transfer between HSA and aztreonam. Site marker displacement experimental conclusion suggested the

SUBMITTER: Sabour AA 

PROVIDER: S-EPMC9698515 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature