Inflammatory Cytokines That Enhance Antigen Responsiveness of Naive CD8⁺ T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation.

ABSTRACT: Naïve CD8⁺ T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such 'cytokine priming' can promote the activation of potentially autoreactive and antitumor CD8⁺ T cells by weak tissue antigens and tumor antigens. To elucidate the molecular mechanisms of cytokine priming, naïve PMEL-1 TCR transgenic CD8⁺ T lymphocytes were stimulated with IL-15 and IL-21, and chromatin accessibility was assessed using the assay for transposase-accessible chromatin (ATAC) sequencing. PMEL-1 cells stimulated by the cognate antigenic peptide mgp100_25-33 served as controls. Cytokine-primed cells showed a limited number of opening and closing chromatin accessibility peaks compared to antigen-stimu

SUBMITTER: Quenum AJI 

PROVIDER: S-EPMC9698886 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature