High Glucose-Induced Cardiomyocyte Damage Involves Interplay between Endothelin ET-1/ET_A/ET_B Receptor and mTOR Pathway.

ABSTRACT: Patients with type two diabetes mellitus (T2DM) are at increased risk for cardiovascular diseases. Impairments of endothelin-1 (ET-1) signaling and mTOR pathway have been implicated in diabetic cardiomyopathies. However, the molecular interplay between the ET-1 and mTOR pathway under high glucose (HG) conditions in H9c2 cardiomyoblasts has not been investigated. We employed MTT assay, qPCR, western blotting, fluorescence assays, and confocal microscopy to assess the oxidative stress and mitochondrial damage under hyperglycemic conditions in H9c2 cells. Our results showed that HG-induced cellular stress leads to a significant decline in cell survival and an impairment in the activation of ET_A-R/ET_B-R and the mTOR main components, Raptor and Rictor. These changes induce

SUBMITTER: Pandey S 

PROVIDER: S-EPMC9699386 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature