AIE+ESIPT Active Hydroxybenzothiazole for Intracellular Detection of Cu²⁺: Anticancer and Anticounterfeiting Applications.

ABSTRACT: Here, in the present work, a new hydroxybenzothiazole derivative (HBT 2) with AIE+ESIPT features was synthesized by Suzuki-Miyora coupling of HBT 1 with 4-formylphenylboronic acid. The AIE and ESIPT features were confirmed by optical, microscopic (AFM) and dynamic light scattering (DLS) techniques. The yellow fluorescent aggregates of HBT 2 can specifically detect Cu²⁺/Cu⁺ ions with limits of detection as low as 250 nM and 69 nM. The Job's plot revealed the formation of a 1:1 complex. The Cu²⁺ complexation was further confirmed by optical, NMR, AFM and DLS techniques. HBT 2 was also used for the detection of Cu²⁺ ions in real water samples collected from different regions of Punjab. HBT 2 was successfully used for the bio-imaging of Cu²⁺ ions in live A549 and its anticancer activity was checked on different cancer cell lines, such as MG63, and HeLa, and normal cell lines such as L929. We successfully utilized HBT 2 to develop security labels for anticounterfeiting applications.

SUBMITTER: Kaur R 

PROVIDER: S-EPMC9699452 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature