Temporal in vivo platelet labeling in mice reveals age-dependent receptor expression and conservation of specific mRNAs.

ABSTRACT: The proportion of young platelets, also known as newly formed or reticulated, within the overall platelet population has been clinically correlated with adverse cardiovascular outcomes. However, our understanding of this is incomplete because of limitations in the technical approaches available to study platelets of different ages. In this study, we have developed and validated an in vivo temporal labeling approach using injectable fluorescent antiplatelet antibodies to subdivide platelets by age and assess differences in functional and molecular characteristics. With this approach, we found that young platelets (<24 hours old) in comparison with older platelets respond to stimuli with greater calcium flux and degranulation and contribute more to the formation of thrombi in vitro and in vi

SUBMITTER: Armstrong PC 

PROVIDER: S-EPMC9699941 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature