Identification and validation of autophagy-related genes during osteogenic differentiation of bone marrow mesenchymal stem cells.

ABSTRACT:

Objectives

Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is an essential stage in bone formation. Autophagy plays a pivotal role in the self-renewal potential and pluripotency of stem cells. This study aimed to explore the function of autophagy-related genes during osteogenic differentiation of BMSCs.

Materials and methods

The differentially expressed autophagy-related genes (ARGs) were obtained from the GEO and HADb databases. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using R software. The PPI and hub gene mining networks were constructed using the STRING database and Cytoscape. Finally, the RT-qPCR was conducted to validate the expression level of ARGs in BMSCs.

Results

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SUBMITTER: Li Y 

PROVIDER: S-EPMC9699953 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature