Lipopolysaccharide enhances HSV-1 replication and inflammatory factor release in the ARPE-19 cells.

ABSTRACT:

Purpose

During acute retinal necrosis (ARN), retinal pigment epithelial (RPE) cells could be stimulated by both herpes simplex virus (HSV) and lipopolysaccharide (LPS). We aim to investigate the impact of LPS on HSV-1 infection and inflammatory factors in human retinal pigment epithelial cell lines (ARPE-19 cells).

Methods

ARPE-19 cells were infected by HSV-1F strain and HSVg4 strain, a modified HSV strain with GFP genes cloned in, for 1 h. Different concentrations of LPS were added. Green fluorescence protein (GFP) of HSVg4 and the infected cell protein 4 (ICP4) expression were observed. Cell culture supernatants were collected to detect 34 kinds of related cytokines and chemokines by multiplex immunoassay assay.

Results

Under LPS treatment, the cytopathic eff

SUBMITTER: Duan F 

PROVIDER: S-EPMC9699980 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature