Impacts of neoadjuvant chemoradiotherapy on the immune landscape of esophageal squamous cell carcinoma.

ABSTRACT:

Background

Neoadjuvant chemoradiotherapy (neoCRT) followed by surgery is the most common approach for locally advanced resectable esophageal squamous cell carcinoma (ESCC) patients. How neoCRT impacts ESCC tumor immune microenvironment (TIME) has not been fully understood.

Methods

Single-cell RNA sequencing (scRNA-seq) was conducted to examine the neoCRT-driven cellular and molecular dynamics in 8 pre- and 7 post-neoCRT ESCC samples from 8 male patients.

Findings

scRNA-seq data of about 112,000 cells were obtained. Expression programs of cell cycle, epithelium development, immune response, and extracellular structure in pre-treatment tumor cells were related to neoCRT response. Spearman correlation between CD8⁺ T cells' cytotoxicity and expression of checkp

SUBMITTER: Wen J 

PROVIDER: S-EPMC9699982 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature