BMP4/SMAD8 signaling pathway regulated granular cell proliferation to promote follicle development in Wanxi white goose.

ABSTRACT: Granular cells proliferation in goose regulated by bone morphogenetic proteins (BMPs) signaling pathway is still unknown. In this experiment, BMPs and their receptor, and receptor activated mothers against decapentaplegic homologs (SMADs) were quantitatively expressed in granular cell layer of pre-hierarchycal and hierarchycal follicles in Wanxi White goose. The screened BMP was then used for construction of overexpressed and knockdown vectors and transfected into granular cells of goose to assess the cell proliferation and apoptosis. Granular cells with BMP-overexpressed were then used for ChIP-Seq analysis to elucidate the molecular mechanism of BMP affecting granular cell proliferation. The results showed that the mRNA expression of BMP4 was significantly expressed in pre-hierarchical f

SUBMITTER: Wei C 

PROVIDER: S-EPMC9700011 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature