Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats.

ABSTRACT: Exosome-derived long non-coding RNAs (lncRNAs) are extensively engaged in recovery and repair of the injured spinal cord, through different mechanisms. However, to date no study has systematically evaluated the differentially expressed lncRNAs involved in the development of spinal cord injury. Thus, the aim of this study was to identify key circulating exosome-derived lncRNAs in a rat model of spinal cord injury and investigate their potential actions. To this end, we established a rat model of spinal cord hemisection. Circulating exosomes were extracted from blood samples from spinal cord injury and control (sham) rats and further identified through Western blotting and electron microscopy. RNA was isolated from the exosomes and sequenced. The enrichment analysis demonstrated that there w

SUBMITTER: Li JA 

PROVIDER: S-EPMC9700090 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature