Melatonin suppresses serum starvation-induced autophagy of ovarian granulosa cells in premature ovarian insufficiency.

ABSTRACT:

Objectives

Premature ovarian insufficiency (POI) refers to the decline and cessation of ovarian functions in women under 40 years of age. Melatonin (MT) acts as a protective for the ovary. This study elucidated the role of MT in autophagy of granulosa cells (GCs) in POI via modulating the phosphatidylinositol-3-kinase (PI3K)-Akt-mammalian target of rapamycin (mTOR) pathway.

Methods

The expression levels of microRNA (miR)-15a-5p, signal transducer and activator of transcription 3 (Stat3), and relevant hormones in the clinically collected serum samples of POI patients and healthy controls were examined. Human ovarian granulosa-like tumor cells (KGN) underwent serum starvation (SS) treatment to induce POI cell models and then received MT treatment. The expression levels of miR-

SUBMITTER: Wu D 

PROVIDER: S-EPMC9700896 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature