Noninvasive detection of pancreatic ductal adenocarcinoma using the methylation signature of circulating tumour DNA.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) has the lowest overall survival rate primarily due to the late onset of symptoms and rapid progression. Reliable and accurate tests for early detection are lacking. We aimed to develop a noninvasive test for early PDAC detection by capturing the circulating tumour DNA (ctDNA) methylation signature in blood.

Methods

Genome-wide methylation profiles were generated from PDAC and nonmalignant tissues and plasma. Methylation haplotype blocks (MHBs) were examined to discover de novo PDAC markers. They were combined with multiple cancer markers and screened for PDAC classification accuracy. The most accurate markers were used to develop PDACatch, a targeted methylation sequencing assay. PDACatch was applied to additional PDAC and h

SUBMITTER: Wu H 

PROVIDER: S-EPMC9701032 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature