Genes related to allergen exposure in allergic rhinitis: a gene-chip-based study in a mouse model.

ABSTRACT:

Background

The typical clinical symptoms of allergic rhinitis (AR) are known to be associated with allergen exposure; however, the underlying mechanisms are not fully understood. We wanted to gain a comprehensive view of the molecular mechanisms related to allergen exposure in a well-controlled mouse model of AR.

Methods

An OVA-induced AR model was developed. Two hours and 4 weeks after the last OVA challenge, AR symptoms and local immune responses were assessed. At the same time, differentially expressed genes (DEG) in nasal mucosa were identified by gene expression microarray and further analyzed by bioinformatics methods. Verification of DEG was done by quantitative RT-PCR and immunohistochemistry.

Results

The number of nasal rubbings and sneezes, serum OVA-specifi

SUBMITTER: Wang M 

PROVIDER: S-EPMC9701046 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature